Published scientific research

Schwartz, A  et al.  (2012). Ozono therapy and its Scientific Foundations. Revista Española de Ozonoterapia. Vol. 2, nº 1, pp. 199-232.

“This document, based on the latest books and scientific articles on the subject, updates the recent findings that justify, from the scientific point of view, the medical use of ozone (…) s with any healing technique, ozone therapy is dependent on the dosage. It is important to understand that in clinical practice the concentrations of ozone are lower than the toxic levels by several orders of magnitude.”

On October 27, 2021 in the Medline database (PubMed) there were 4024 documents related to ozone therapy, of which 301 were clinical trials, 207 randomized controlled trials, 55 of systematic review and 33 meta-analysis studies, which supported the use of ozone in medicine.

In addition, in the database of clinical studies at the US National Institutes of Health there were 61 studies registered for ozone therapy, of which their status was: 3 ended, 25 completed, 3 unenrolled active, 13 recruiting.


Ozone therapy and Covid-19

The World Health Organization (WHO), in February 2021 officially stated: “Currently, there are no vaccines or specific pharmaceutical treatments available for COVID-19.”

To combat the pandemic, the WHO have called “countries to take urgent and aggressive action”; and stating that “this is not just a public health crisis, it is a crisis that will touch every sector – so every sector and every individual must be involved in the fight.”

ISCO3 (International Scientific Committee of Ozone Therapy) as part of the world health sector pioneered, the month after the WHO call, proposed in a pioneering way, the potential use of ozone therapy as a complementary therapy, based exclusively on available scientific data.


Proposal published in the paper “Potential use of ozone in SARS-CoV-2 / COVID-19” (March 14, 2020)


Since then, on the committee’s website, all the research on ozone therapy and COVID-19 that has been published in the world, has been compiled and updated, under the following titles:

  • Clinical Trials Follow-up Preliminary Results O2/O3 & COVID-19. P. 1
  • ISCO3 Recommendations Use O2/O3 in COVID-19. P. 2
  • ISCO3 Outcomes Use O2/O3 & COVID-19. P. 3
  • Ozone Therapy Support papers on SARS-CoV-2 / COVID-19. P. 4.


The main results of the studies indicate that ozone decreases the inflammation indices and the time of assisted breathing, improves oxygen saturation and makes CRP negative in shorter periods. Increases oxygen saturation. And it could reduce mortality.


The routes of application have been ozonized saline solution, major autohemotherapy, and rectal insufflation.


Ozonized Saline Solution

“Ozonized Saline Solution (O3SS) is a widespread practice in Russia and developed by the Russian school of ozone therapy in the city of Nizhny Novgorod (Volga Federal District).” With enough success it has started to be implemented in the last decade in several countries outside of Russia.


The Ozone Saline Solution is already an application recommended by the Madrid Declaration on Ozone Therapy. Item 3.1.3


Ozonized saline solution and Covid-19

Among the research advances that medical ozone has had, the ozonized saline solution – a route of application of ozone therapy – has stood out. Its use, as a complementary therapy, in patients hospitalized for Covid-19 has played a highly significant role in overcoming the disease.


The results of the research carried out at the Viamed Virgen de la Paloma Hospital in Madrid (Spain), demonstrated the efficiency of the ozonized saline solution.

The research has been already published.


Schwartz Adriana et al. “Complementary application of the ozonized saline solution in mild and severe patients with pneumonia COVID-19: A non-randomized pilot study.” J Pharm Pharmacogn Res 9(2): 126–142.

A synthesis of the research results can be found in


Ozonized Saline Solution in the WHO library

The WHO (World Health Organization) has published in its virtual library “COVID-19 – Global literature on coronavirus disease” the three following investigations on Ozonized Saline Solution and COVID-19.


Schwartz, Adriana. et al. Complementary application of the ozonized saline solution in mild and severe patients with pneumonia COVID-19: A non-randomized pilot study.

Access to full paper

Martínez-Sánchez, G. et al. Potential Cytoprotective Activity of Ozone Therapy in SARS-CoV-2/COVID-19.

Access to full paper

Schwartz Adriana et al. Potential use of ozone in SARS-CoV-2 / COVID-19. ISCO3

Access to full paper 

Pacheco Cárdenas Iván Alexis et al. (2021). Ozone Therapy In the Prevention and Treatment Of Long-Term Health Consequences of OF COVID-19 Patients. Cases Report.

Ozone therapy and post-COVID-19 sequelae

The first researches on ozone treatment in the reversal of the sequels of covid-19, has been already issued.


Fernando C. Dotta Barros et al. Persistent Anosmia after COVID-19 Infection: Treatment Options According to BDORT. Acupuncture and Electro-Therapeutics Research; 46(1):33-34, 2021.


Pacheco Cárdenas Iván Alexis et al. (2021). La ozonoterapia en la prevención y tratamiento de las secuelas de pacientes de Covid-19. Reporte de casos.. Ozone Therapy Global Journal Vol. 11, nº 1, pp 125-139.


Tirelli U.,et al. Fatigue in post-acute sequelae of SARS-CoV2 (PASC) treated with oxygen-ozone autohemotherapy – preliminary results on 100 patients. Eur Rev Med Pharmacol Sci. 2021 Sep; 25(18):5871-5875. doi: 10.26355/eurrev_202109_26809.


Ozone therapy: experimental treatment to treat COVID-19


Ozone therapists from different parts of the world, as indicated above, have published the results of the application of ozone therapy in COVID-19 patients in scientific journals.


However, it is convenient to specify that for the treatment of COVID-19 “ozone cannot be considered an already proven therapy, but one more experimental treatment, like so many others that have been administered during the pandemic with few specific studies behind it, considering that its mechanism of action in other pathologies can be useful in coronavirus. This is common in a new disease for which there is no known treatment, and especially when the patient is in a situation with little chance of survival. The paucity of studies on a safe treatment with reasonable evidence of efficacy should encourage further research until it can be known whether it works or not.”


It is worth remembering “that confusing scarcity of evidence with no evidence is a serious epistemological error. To lightly disqualify a whole line of research under the concept of ‘pseudo-therapy’, without thoroughly reviewing and entering into debate with the studies published on the matter, is an irresponsibility that can condemn countless present and future patients.”

Pireto Valient, Luis et al. Who decides what a pseudo-therapy is? Evidence vs hasty opinions on ozone



This content is also available in: Spanish

Únete a nuestra comunidad de seguidores Ozonoterapeutas


  • Recibirás en tu correo contenidos exclusivos


  • Estarás al día en nuevas noticias de Ozonoterapia


  • Te informaremos de cuándo son nuestros cursos para Ozonoterapeutas


  • Te informaremos sobre congresos


  • Sabrás cuándo ha salido artículos de Ozonoterapia en revistas científicas


  • Te informaremos de forma prioritaria de los nuevos núneros de nuestra revista científica «Ozone Therapy Global Journal


  • Y mucho más…

Gracias por unirte a nuestra comunidad.

En breve recibirás un correo para confirmar tu suscripción. Revisa tu bandeja de entrada y si en unos minutos no ves un mensaje nuestro, mira en la bandeja de Spam